Anticorps Polyclonal de lapin anti-ZNF394

ZNF394 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 25785-1-AP

Synonymes

ZKSCAN14

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Galerie de données de validation

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  WB analysis of mouse brain using 25785-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 25785-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25785-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25785-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 25785-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 25785-1-AP (ZNF394 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, tissu cérébral humain fœtal, tissu splénique de souris
• Résultats positifs en IHC: tissu de carcinome urothélial humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25785-1-AP cible ZNF394 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF394 Protéine recombinante Ag22655
• Nom complet: zinc finger protein 394
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC051847
• Symbole du gène: ZNF394
• Identification du gène (NCBI): 84124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNF394 (zinc finger protein 394) is a C2H2-type zinc finger protein belonging to the Krüppel C2H2-type zinc finger protein family. The protein contains three distinct domains: the N-terminus has two domains, LER and KRAB, and the C-terminus has seven C2H2 zinc finger domains. ZNF394 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZNF394, together with ZIM3, plays a key role in human embryonic development at the 8-cell stage by inhibiting the expression of genes specific to the 4-cell stage, thereby promoting embryonic development.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Simone (Verified Customer) (04-25-2022)

From 6 well plate pellet, lysed with 1ml NP40 buffer

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells

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