Anticorps Polyclonal de lapin anti-ZNF395

• Phare
• Validé par KD/KO

ZNF395 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11759-1-PBS

Synonymes

HD-regulating factor 2, HDBP-2, HDBP2, HDBP 2, HD regulating factor 2

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Galerie de données de validation

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  WB analysis of human brain using 11759-1-AP (same clone as 11759-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11759-1-AP (same clone as 11759-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11759-1-AP (same clone as 11759-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11759-1-AP (same clone as 11759-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11759-1-AP (ZNF395 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11759-1-AP (same clone as 11759-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZNF395 antibody (11759-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11759-1-PBS cible ZNF395 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF395 Protéine recombinante Ag2355
• Nom complet: zinc finger protein 395
• Masse moléculaire calculée: 513 aa, 55 kDa
• Poids moléculaire observé: 62 kDa, 72 kDa
• Numéro d’acquisition GenBank: BC001237
• Symbole du gène: ZNF395
• Identification du gène (NCBI): 55893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].

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