Anticorps Polyclonal de lapin anti-ZNF618

• Phare
• Validé par KD/KO

ZNF618 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31972-1-PBS

Synonymes

KIAA1952, Zinc finger protein 618

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Galerie de données de validation

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  WB analysis using 31972-1-AP (same clone as 31972-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31972-1-AP (ZNF618 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 31972-1-AP (same clone as 31972-1-PBS)

  WB result of ZNF618 antibody (31972-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF618 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 31972-1-AP (same clone as 31972-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 31972-1-AP (same clone as 31972-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 31972-1-AP (same clone as 31972-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF618 antibody (31972-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31972-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31972-1-PBS cible ZNF618 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF618 Protéine recombinante Ag36487
• Nom complet: zinc finger protein 618
• Masse moléculaire calculée: 105kDa，954aa
• Poids moléculaire observé: 100-113 kDa
• Numéro d’acquisition GenBank: NM_001318042.1
• Symbole du gène: ZNF618
• Identification du gène (NCBI): 114991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.

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