Anticorps Recombinant de lapin anti-ZNF618

• Phare
• Validé par KD/KO

ZNF618 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242566D9

N° de cat : 84998-1-PBS

Synonymes

242566D9, KIAA1952, Zinc finger protein 618

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Galerie de données de validation

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  WB analysis using 84998-1-RR (same clone as 84998-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84998-1-RR (ZNF618 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 84998-1-RR (same clone as 84998-1-PBS)

  WB result of ZNF618 antibody (84998-1-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF618 transfected SH-SY5Y cells. This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84998-1-RR (same clone as 84998-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84998-1-RR (ZNF618 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84998-1-RR (same clone as 84998-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF618 antibody (84998-1-RR, Clone: 242566D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84998-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01753-2

  Cytometric bead array standard curve of MP01753-2, ZNF618 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84998-1-PBS. Detection antibody: 84998-2-PBS. Standard: Ag36487. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 84998-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84998-1-RR against Human ZNF618 were performed. The affinity constant is 1.64 nM.

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Informations sur le produit

84998-1-PBS cible ZNF618 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZNF618 Protéine recombinante Ag36487
• Nom complet: zinc finger protein 618
• Masse moléculaire calculée: 105kDa，954aa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: NM_001318042.1
• Symbole du gène: ZNF618
• Identification du gène (NCBI): 114991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.

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