Anticorps Polyclonal de lapin anti-ZNF638

ZNF638 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Avis (3)

N° de cat : 31300-1-AP

Synonymes

CTCL associated antigen se33 1, NP220, Nuclear protein 220, ZFML, zinc finger protein 638

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Galerie de données de validation

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  WB analysis using 31300-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31300-1-AP (ZNF638 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 31300-1-AP

  IP result of anti-ZNF638 (IP:31300-1-AP, 4ug; Detection:31300-1-AP 1:3000) with Jurkat cells lysate 1360 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31300-1-AP cible ZNF638 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF638 Protéine recombinante Ag35296
• Nom complet: zinc finger protein 638
• Masse moléculaire calculée: 221kDa
• Poids moléculaire observé: 270-300 kDa
• Numéro d’acquisition GenBank: NM_014497.4
• Symbole du gène: ZNF638
• Identification du gène (NCBI): 27332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNF638 (Zinc finger protein 638) is also named as NP220 and ZFML. ZNF638 is a newly discovered member of zinc finger protein family for adipocyte function in vitro. And ZNF638regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner (PMID: 38211696). ZNF638 plays a role as a transcriptional coregulator of adipocyte differentiation via induction of PPARγ in cooperation with CCAAT/enhancer binding proteins (C/EBPs) (PMID: 25024404). ZNF638 is localized in nuclear bodies enriched with splicing factors (PMID: 25024404). The ZNF638 protein is modified and exhibits a molecular weight of ~300 kDa (PMID: 33040080)

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-29-2025)

Antibody recognise a band of the expected size with some other prominent, likely non-specific ones

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

ZNF638 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:31300-1-AP)

FH

Tsimafei (Verified Customer) (08-07-2024)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

ZNF638 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:31300-1-AP)

FH

Julia (Verified Customer) (08-07-2024)

Band below 250 kDa marker is likely the Znf639 (~221kDa), there is also strong signal between 100-50kDa. I used 30ug of mESC protein extract.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESC

ZNF638 Antibody Western Blot validation (1:5000 dilution) in mESC (Cat no:31300-1-AP)

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