Anticorps Polyclonal de lapin anti-ZNF703

ZNF703 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 21075-1-AP

Synonymes

ZPO1, ZNF503L, Zinc finger elbow-related proline domain protein 1, ZEPPO1

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 21075-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21075-1-AP (ZNF703 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21075-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21075-1-AP (ZNF703 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21075-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21075-1-AP (ZNF703 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse intestine using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 21075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21075-1-AP (ZNF703 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cérébral humain, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané de rat, tissu cutané de souris, tissu de cervelet de rat, tissu de cervelet de souris, tissu de mélanome malin humain, tissu d'intestin grêle de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

21075-1-AP cible ZNF703 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF703 Protéine recombinante Ag15242
• Nom complet: zinc finger protein 703
• Masse moléculaire calculée: 590 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC032534
• Symbole du gène: ZNF703
• Identification du gène (NCBI): 80139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.; Authors - Xiaohui Zhang

{{ptg:RelatedPrimaryAntibodies}}