Anticorps Monoclonal anti-ZNF746

ZNF746 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A12C5

N° de cat : 67463-1-PBS

Synonymes

FLJ31413, PARIS, Parkin-interacting substrate, Zinc finger protein 746

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Galerie de données de validation

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  WB analysis of HEK-293 using 67463-1-Ig (same clone as 67463-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67463-1-Ig (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67463-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67463-1-Ig (same clone as 67463-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67463-1-Ig (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67463-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67463-1-PBS cible ZNF746 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZNF746 Protéine recombinante Ag21582
• Nom complet: zinc finger protein 746
• Masse moléculaire calculée: 644 aa, 69 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC068505
• Symbole du gène: ZNF746
• Identification du gène (NCBI): 155061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN: its accumulation due to the absence of PRKN, followed by up-regulation of PPARGC1A, could lead to the selective loss of dopamine neurons in the substantia nigra(PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).

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