Anticorps Polyclonal de lapin anti-ZNHIT1

ZNHIT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16595-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16595-1-AP (same clone as 16595-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16595-1-AP (ZNHIT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 16595-1-AP (same clone as 16595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16595-1-AP (ZNHIT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 16595-1-AP (same clone as 16595-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16595-1-AP (same clone as 16595-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZNHIT1 antibody (16595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16595-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16595-1-PBS cible ZNHIT1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNHIT1 Protéine recombinante Ag9741
• Nom complet: zinc finger, HIT type 1
• Masse moléculaire calculée: 154 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC017333
• Symbole du gène: ZNHIT1
• Identification du gène (NCBI): 10467
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZNHIT1(Zinc finger HIT domain containing protein 1), aslo named CGBP1 (Cyclin G1 binding protein 1), is a subunit of the SNF-2 related CBP activator protein (SRCAP) complex, which can remodel chromatin by incorporating the histone variant H2A.Z into nucleosomes(PMID: 15647280). ZNHIT1 also can bind to myogenin promoter and mediate H2A.Z incorporation for its expression(PMID: 20473270). ZNHIT1 overexpression induces apoptosis and represses proliferation and invasion of breast cancer cells(PMID: 30928405).

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