Anticorps Monoclonal anti-ZNHIT3

ZNHIT3 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C7A3

N° de cat : 68331-1-Ig

Synonymes

HNF 4a coactivator, TR interacting protein 3, TRIP 3, TRIP3, zinc finger, HIT type 3, ZNHIT3

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Galerie de données de validation

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  WB analysis of hTERT-RPE1 using 68331-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68331-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 68331-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 68331-1-Ig (ZNHIT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68331-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ZNHIT3 antibody (68331-1-Ig, Clone: 1C7A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules hTERT-RPE1, cellules Jurkat, cellules MOLT-4, cellules T-47D
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68331-1-Ig cible ZNHIT3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZNHIT3 Protéine recombinante Ag29872
• Nom complet: zinc finger, HIT type 3
• Masse moléculaire calculée: 155 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC017931
• Symbole du gène: ZNHIT3
• Identification du gène (NCBI): 9326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNHIT3, also named TRIP3, belongs to the zinc finger HIT (Zf-HIT) domain-containing proteins family. ZNHIT3 encodes a nuclear zinc finger protein previously implicated in transcriptional regulation and small nucleolar ribonucleoprotein particle assembly and thus possibly to pre-ribosomal RNA processing (PMID: 28335020). ZNHIT3 contains at least two domains: a ZN-HIT domain that consists of a double zinc-finger, probably involved in protein−protein interaction, and a PAC-HIT domain that folds into a clamp able to trap an α-helix, here again of about 20 residues, located in the sequence of NUFIP1 (PMID: 35315277). ZNHIT3 has 2 isoforms produced by alternative splicing.

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