Anticorps Polyclonal de lapin anti-ZNRF2-Specific

ZNRF2-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20200-1-AP

Synonymes

Protein Ells2, RING finger protein 202, RNF202, zinc and ring finger 2, Zinc/RING finger protein 2, ZNRF2, ZNRF2-Specific

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Galerie de données de validation

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  WB analysis using 20200-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20200-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20200-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20200-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20200-1-AP (ZNRF2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris, tissu rénal de souris, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

20200-1-AP cible ZNRF2-Specific dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: zinc and ring finger 2
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: NM_147128
• Symbole du gène: ZNRF2
• Identification du gène (NCBI): 223082
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNRF2 also named as RNF202, may play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. The antibody is specific to ZNRF2.

Publications

Species	Application	Title
rat	WB	Exp Neurol; ZNRF2 attenuates focal cerebral ischemia/reperfusion injury in rats by inhibiting mTORC1-mediated autophagy.; Authors - Chao Gu
human	WB	Hum Cell; ZNRF2 as an oncogene is transcriptionally regulated by CREB1 in breast cancer models; Authors - Jin-Tao Liu
human	WB	J Cell Mol Med; A pancancer analysis of the oncogenic role of ZNRF2 in human tumours; Authors - Fujie Shi ; KD Validated