Anticorps Recombinant de lapin anti-ZO-2

• Phare
• Validé par KD/KO

ZO-2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230474D1

N° de cat : 83111-1-PBS

Synonymes

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

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Galerie de données de validation

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  WB analysis using 83111-1-RR (same clone as 83111-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83111-1-RR (ZO-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83111-1-RR (same clone as 83111-1-PBS)

  WB result of ZO-2 antibody (83111-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells. This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83111-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83111-1-RR against Human ZO-2 were performed. The affinity constant is 30.8 pM.

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Informations sur le produit

83111-1-PBS cible ZO-2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZO-2 Protéine recombinante Ag13534
• Nom complet: tight junction protein 2 (zona occludens 2)
• Masse moléculaire calculée: 134 kDa
• Poids moléculaire observé: ~150 kDa
• Numéro d’acquisition GenBank: BC027592
• Symbole du gène: TJP2
• Identification du gène (NCBI): 9414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

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