Anticorps Polyclonal de lapin anti-ZO-3

ZO-3 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22116-1-PBS

Synonymes

Tight junction protein 3, Tight junction protein ZO-3, TJP3, ZO3, Zona occludens protein 3

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Galerie de données de validation

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  WB analysis of MCF-7 using 22116-1-AP (same clone as 22116-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

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  WB analysis using 22116-1-AP (same clone as 22116-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 22116-1-AP (same clone as 22116-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

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  IP experiment of HT-29 using 22116-1-AP (same clone as 22116-1-PBS)

  IP result of anti-ZO-3 (IP:22116-1-AP, 4ug; Detection:22116-1-AP 1:1000) with HT-29 cells lysate 1760 ug. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22116-1-PBS cible ZO-3 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZO-3 Protéine recombinante Ag17495
• Nom complet: tight junction protein 3 (zona occludens 3)
• Masse moléculaire calculée: 952 aa, 105 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC108906
• Symbole du gène: ZO-3
• Identification du gène (NCBI): 27134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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