Anticorps Recombinant de lapin anti-ZP3

ZP3 Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242729A8

N° de cat : 85084-1-PBS

Synonymes

242729A8, Processed zona pellucida sperm-binding protein 3, Zona pellucida sperm-binding protein 3, Zp 3, Zp-3

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Galerie de données de validation

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  WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 85084-1-RR (ZP3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 85084-1-RR (ZP3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01801-2

  Cytometric bead array standard curve of MP01801-2, ZP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85084-1-PBS. Detection antibody: 85084-2-PBS. Standard: Ag15791. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85084-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85084-1-RR against Human ZP3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85084-1-PBS cible ZP3 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZP3 Protéine recombinante Ag15791
• Nom complet: zona pellucida glycoprotein 3 (sperm receptor)
• Masse moléculaire calculée: 373 aa, 41 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC113949
• Symbole du gène: ZP3
• Identification du gène (NCBI): 7784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).

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