Anticorps Polyclonal de lapin anti-ZRANB2

• Phare
• Validé par KD/KO

ZRANB2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 24816-1-AP

Synonymes

Zinc finger protein 265, Zinc finger, splicing, ZIS, ZIS1, ZIS2, ZNF265, ZRANB2

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 24816-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24816-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

24816-1-AP cible ZRANB2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZRANB2 Protéine recombinante Ag20487
• Nom complet: zinc finger, RAN-binding domain containing 2
• Masse moléculaire calculée: 330 aa, 37 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC039814
• Symbole du gène: ZRANB2
• Identification du gène (NCBI): 9406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZRANB2, also named as ZIS and ZNF265, is a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. It may interfere with constitutive 5'-splice site selection. The calculated MW of ZRANB2 is 330aa, 37 kDa or 320aa, 36 kDa. We always got the ~48 kDa in our detection. The immunogen of this antibody is 1-241aa. No other protein can be recognized by this antibody from the sequence BLAST results.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.; Authors - Iris Tanaka
human	WB	Aging (Albany NY); LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2.; Authors - Xin Jiang
human	WB,IHC	J Exp Clin Cancer Res; ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.; Authors - Xiaozhi Li ; KD Validated