Anticorps Recombinant de lapin anti-Alpha Actinin

Alpha Actinin Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250833F4

N° de cat : 86340-2-PBS

Synonymes

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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Galerie de données de validation

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  WB analysis using 86340-2-RR (same clone as 86340-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86340-2-RR (Alpha Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  Sample test of MP02360-1

  The mean Alpha Actinin concentration was determined to be 254.6 ng/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86340-2-RR (Alpha Actinin antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86340-2-RR (Alpha Actinin antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (86340-2-RR, Clone: 250833F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (86340-2-RR, Clone: 250833F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 86340-2-RR (same clone as 86340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Alpha Actinin antibody (86340-2-RR, Clone: 250833F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02360-1

  Cytometric bead array standard curve of MP02360-1, Alpha Actinin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86340-2-PBS. Detection antibody: 86340-1-PBS. Standard: Ag1859. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86340-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86340-2-RR against Human Alpha Actinin were performed. The affinity constant is 1.94 nM.

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Informations sur le produit

86340-2-PBS cible Alpha Actinin dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Alpha Actinin Protéine recombinante Ag1859
• Nom complet: actinin, alpha 1
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC015766
• Symbole du gène: Alpha Actinin
• Identification du gène (NCBI): 87
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

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