Anticorps Monoclonal anti-androgen receptor

androgen receptor Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ChIP, Indirect ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1F7C12

N° de cat : 66747-1-PBS

Synonymes

androgen receptor,AR, AR, 1F7C12, DHTR, NR3C4

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Galerie de données de validation

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  WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

  PC-3(AR-), DU 145(AR-) and LNCaP (AR+) cell lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66747-1-Ig (same clone as 66747-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66747-1-Ig (same clone as 66747-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of LNCaP using 66747-1-Ig (same clone as 66747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66747-1-PBS in a different storage buffer formulation.

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Applications publiées

• ChIP: See 1 publications below

Informations sur le produit

66747-1-PBS cible androgen receptor dans les applications de WB, IHC, IF/ICC, IF-P, ChIP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: androgen receptor Protéine recombinante Ag17291
• Nom complet: androgen receptor
• Masse moléculaire calculée: 914 aa, 99 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC132975
• Symbole du gène: AR
• Identification du gène (NCBI): 367
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )

Publications

Species	Application	Title
human	ChIP	Sci Rep; Characterization of RNA editing gene APOBEC3C as a candidate tumor suppressor in prostate cancer; Authors - Li-Yang Wang

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