Anticorps Recombinant de lapin anti-androgen receptor

• Phare
• Validé par KD/KO

androgen receptor Recombinant Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4O18

N° de cat : CL488-81844

Synonymes

AR, androgen receptor,AR, NR3C4, DHTR, 4O18

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Galerie de données de validation

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  IF Staining of LNCaP using CL488-81844

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CoraLite® Plus 488 androgen receptor antibody (CL488-81844, Clone: 4O18 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of LNCaP using CL488-81844

  1x10^6 LNCaP cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated androgen receptor Recombinant antibody (CL488-81844, Clone:4O18)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules LNCaP,
• Résultats positifs en FC (Intra): cellules LNCaP,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-81844 cible androgen receptor dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: androgen receptor Protéine recombinante Ag17291
• Nom complet: androgen receptor
• Masse moléculaire calculée: 914 aa, 99 kDa
• Poids moléculaire observé: 110-120 kD
• Numéro d’acquisition GenBank: BC132975
• Symbole du gène: AR
• Identification du gène (NCBI): 367
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Androgen receptor (AR) is a steroid hormone receptor for androgenic hormones such as 17β-Hydroxy-3-oxo-4-androstene and DHT. AR plays a vital role in developing and maintaining male sex phenotypes as well as an additional role in regulating bone metabolism.

1. What is the molecular weight of AR? Are there any isoforms of AR?
The molecular weight of full-length androgen receptor (AR-B) is 110 kDa. An additional variant, AR-A, has an 87 kDa size and lacks the N-terminal 187 amino acids of AR-A (PMID: 8108393). Recently, more splice variants of AR have been discovered, raising protein products of around 80 kDa length (PMID: 19244107), as well as an AR45 variant of 45 kDa size (PMID: 15634333). AR splice variants differ in their cell line-specific expression (PMID: 24570075).

2. What is the subcellular localization of AR?
AR can be present in either or both of the cytoplasm and nucleus. In androgen-deprived cells, AR is found predominantly in the cytoplasm, while stimulation by androgens causes enrichment of androgen-bound AR in the nucleus. AR shuttles between the cytoplasm and nucleus and its phosphorylation state has an impact on subcellular localization (PMID: 16282370).

3. Is AR post-translationally modified?
Post-translational modifications of the AR include phosphorylation, acetylation, methylation, SUMOylation, and ubiquitination (PMID: 21820033). These modifications have an impact on receptor stability, activity, and can change the observed molecular weight of the AR.

4. How to study AR signaling in cell culture?
It is important to control levels of cell stimulation while also looking at AR signaling. Fetal bovine serum (FBS) that is typically used in cell culture contains low levels of 17β-Hydroxy-3-oxo-4-androstene that are enough to stimulate the growth of prostate cells (PMID: 19676093), including the LNCaP cell line that is a commonly used human prostatic carcinoma cell model (PMID: 6831420). One possibility for complete 17β-Hydroxy-3-oxo-4-androstene deprivation is to use charcoal stripped FBS that removes lipophilic agents, including androgens. It is also not recommended to use phenol red in your medium because it is a weak estrogen (PMID: 3458212). Cell stimulation is often conducted by DHT.

5. What is the role of AR in prostate cancer?
AR plays a key role in the development and physiology of the prostate gland, and also cancer progression (PMID: 15082523). Mutations in AR altering ligands have been observed. The progression of the prostate cancer depends on AR activity and therefore blocking AR activity or lowering androgen levels is a key step related to androgen deprivation therapy (ADT).

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