Anticorps Polyclonal de lapin anti-Beta Catenin

• Phare
• Validé par KD/KO

Beta Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

677

Avis (2)

N° de cat : 51067-2-AP

Synonymes

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin

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Galerie de données de validation

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  WB analysis of HeLa using 51067-2-AP

  WB result of Beta Catenin antibody (51067-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Catenin transfected HeLa cells.

• 

  WB analysis using 51067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51067-2-AP (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 51067-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 51067-2-AP (b-cat antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 51067-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 51067-2-AP (b-cat antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 51067-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51067-2-AP (b-cat antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 51067-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 51067-2-AP (b-cat antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 51067-2-AP

  IP Result of anti-beta-Catenin (IP:51067-2-AP, 4ug; Detection:51067-2-AP 1:500) with mouse liver tissue lysate 6400ug.

• 

  IHC staining of human colon cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51067-2-AP (beta-Catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51067-2-AP (beta-Catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 51067-2-AP (beta-Catenin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 51067-2-AP (beta-Catenin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51067-2-AP (beta-Catenin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51067-2-AP (beta-Catenin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51067-2-AP (beta-Catenin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51067-2-AP (beta-Catenin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 51067-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of T-47D using 51067-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 51067-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 51067-2-AP (beta-Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 51067-2-AP

  Immunofluorescent analysis of HepG2 cells, using CTNNB1 antibody 51067-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of MCF-7 using 51067-2-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Beta Catenin (51067-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 51067-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Beta Catenin (51067-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C6, cellules HEK-293, cellules HeLa, cellules MCF-7, cellules NIH/3T3, cellules SH-SY5Y, tissu hépatique de rat, tissu hépatique de souris, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de côlon de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules HeLa, cellules HepG2, cellules T-47D, tissu testiculaire de souris
• Résultats positifs en cytométrie: cellules MCF-7, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 587 publications below
• IHC: See 97 publications below
• IF: See 144 publications below
• IP: See 9 publications below
• CoIP: See 9 publications below
• ChIP: See 5 publications below

Informations sur le produit

51067-2-AP cible Beta Catenin dans les applications de WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, canin, Chèvre, Humain, poisson-zèbre, porc, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: catenin (cadherin-associated protein), beta 1, 88kDa
• Masse moléculaire calculée: 781 aa, 86 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC058926
• Symbole du gène: CTNNB1
• Identification du gène (NCBI): 1499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publications

Species	Application	Title
mouse	WB,IF,IP,IHC	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	IF	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Hepatology; Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.; Authors - Meng Lu
rat	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
mouse	WB	Hepatology; Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.; Authors - Sheng-Xian Yuan
human	WB	Nat Commun; CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.; Authors - Yuan Fang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
• Cell Tissue Type: Bone Marrow MSCs

FH

Alzbeta (Verified Customer) (03-13-2019)

This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Primary rat neonatal cardiomyocytes

Beta Catenin Antibody Immunofluorescence, validation (1:100 dilution) in Primary rat neonatal cardiomyocytes (Cat no:51067-2-AP)

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