Anticorps Monoclonal anti-Band 3/AE 1

Band 3/AE 1 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E5C1

N° de cat : 66984-1-PBS

Synonymes

band 3, band 3/ AE1, SLC4A1, 1E5C1, AE 1

---

Galerie de données de validation

• 

  IHC staining of human placenta using 66984-1-Ig (same clone as 66984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66984-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66984-1-Ig (same clone as 66984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66984-1-Ig (band 3/ AE1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66984-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 66984-1-Ig (same clone as 66984-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3/AE 1 antibody (66984-1-Ig, Clone: 1E5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66984-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 66984-1-Ig (same clone as 66984-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Band 3/AE 1 antibody (66984-1-Ig, Clone: 1E5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66984-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66984-1-PBS cible Band 3/AE 1 dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Band 3/AE 1 Protéine recombinante Ag28058
• Nom complet: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Masse moléculaire calculée: 911 aa, 102 kDa
• Numéro d’acquisition GenBank: BC101570
• Symbole du gène: band 3
• Identification du gène (NCBI): 6521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

{{ptg:RelatedPrimaryAntibodies}}