Anticorps Monoclonal anti-Beta Actin

• Phare
• Validé par KD/KO

Beta Actin Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, poisson-zèbre, rat, singe, souris, Hamster

Applications

WB, IP

Conjugaison

HRP

Publications

50

CloneNo.

2D4H5

N° de cat : HRP-66009

Synonymes

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin

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Galerie de données de validation

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  WB analysis using HRP-66009

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-66009 (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 48 publications below
• IP: See 1 publications below

Informations sur le produit

HRP-66009 cible Beta Actin dans les applications de WB, IP et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, singe, souris, Hamster

• Réactivité: Humain, poisson-zèbre, rat, singe, souris, Hamster
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: actin, beta
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_001101
• Symbole du gène: ACTB
• Identification du gène (NCBI): 60
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.

Publications

Species	Application	Title
mouse	WB	Protein Cell; Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm; Authors - Kuisheng Liu
	WB	Nat Commun; Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice; Authors - Nana Yan
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human,mouse	WB	Nat Commun; SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer.; Authors - Xiaowen Wang
human	WB	Bone Res; Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.; Authors - Dong Wang
mouse	WB	JCI Insight; CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling; Authors - Marta Bueno