Anticorps Polyclonal de lapin anti-APP

APP Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 27320-1-AP

Synonymes

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), Amyloid beta A4 protein, amyloid P, amyloid precursor protein, Amyloid-beta precursor protein, APP, APPI, beta Amyloid, CTFgamma, CVAP, Gamma CTF(50) C31, PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta

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Galerie de données de validation

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  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (beta Amyloid antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (APP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27320-1-AP (APP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 27320-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 27320-1-AP (beta Amyloid antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 27320-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27320-1-AP (beta Amyloid antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules HEK-293T, cellules HeLa, cellules U-251, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

27320-1-AP cible APP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APP Protéine recombinante Ag26299
• Nom complet: amyloid beta (A4) precursor protein
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC004369
• Symbole du gène: APP
• Identification du gène (NCBI): 351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).

Publications

Species	Application	Title
human	WB	Alzheimers Res Ther; Krüppel-like factor 5 accelerates the pathogenesis of Alzheimer's disease via BACE1-mediated APP processing.; Authors - Yaqi Wang