Anticorps Recombinant de lapin anti-TGFBI/BIGH3

TGFBI/BIGH3 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243108H4

N° de cat : 80805-3-PBS

Synonymes

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

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Galerie de données de validation

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  WB analysis of mouse eye using 80805-3-RR (same clone as 80805-3-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  WB analysis of rat eye using 80805-3-RR (same clone as 80805-3-PBS)

  rat eye tissue were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 80805-3-RR (same clone as 80805-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02279-1

  The mean TGFBI/BIGH3 concentration was determined to be 3586.8 ng/mL in human serum.

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  Cytometric bead array standard curve of MP02279-1

  Cytometric bead array standard curve of MP02279-1, TGFBI/BIGH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80805-3-PBS. Detection antibody: 80805-2-PBS. Standard: Eg1233. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 80805-3-RR

  Biolayer interferometry (BLl) kinetic assays of 80805-3-RR against Human TGFBI/BIGH3 were performed. The affinity constant is 2.62 nM.

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Informations sur le produit

80805-3-PBS cible TGFBI/BIGH3 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TGFBI/BIGH3 Protéine recombinante Eg1233
• Nom complet: transforming growth factor, beta-induced, 68kDa
• Masse moléculaire calculée: 75kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: NM_000358.3
• Symbole du gène: TGFBI
• Identification du gène (NCBI): 7045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

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