Anticorps Monoclonal anti-c-MYC

• Phare
• Validé par KD/KO

c-MYC Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

3D9C12

N° de cat : 67447-1-PBS

Synonymes

MYC, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

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Galerie de données de validation

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  WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67447-1-Ig (same clone as 67447-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  FC experiment of NCCIT using 67447-1-Ig (same clone as 67447-1-PBS)

  1x10^6 NCCIT cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67447-1-Ig (same clone as 67447-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67447-1-PBS cible c-MYC dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: c-MYC Protéine recombinante Ag17519
• Nom complet: v-myc myelocytomatosis viral oncogene homolog (avian)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC000141
• Symbole du gène: MYC
• Identification du gène (NCBI): 4609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

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