Anticorps Monoclonal anti-c-MYC

• Phare
• Validé par KD/KO

c-MYC Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(2)

CloneNo.

3D9C12

Avis (1)

N° de cat : CL488-67447

Synonymes

MYC, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

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Galerie de données de validation

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  FC experiment of K-562 using CL488-67447

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human c-MYC (CL488-67447, Clone:3D9C12) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en FC (Intra): cellules K-562

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

CL488-67447 cible c-MYC dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: c-MYC Protéine recombinante Ag17519
• Nom complet: v-myc myelocytomatosis viral oncogene homolog (avian)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC000141
• Symbole du gène: MYC
• Identification du gène (NCBI): 4609
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

Publications

Species	Application	Title
mouse	FC	Int Immunopharmacol; Oxymatrine inhibits melanoma development by modulating the immune microenvironment and targeting the MYC/PD-L1 pathway; Authors - Xin Li
	IF	Nat Commun; Hippo signaling pathway regulates Ebola virus transcription and egress; Authors - Jingjing Liang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lisa (Verified Customer) (04-03-2025)

AB gives a nice signal in GC B cells compared to non-GC B cells

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:125
• Cell Tissue Type: splenocytes

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