Anticorps Monoclonal anti-c-SRC

c-SRC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris, Hamster

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5E10C4

N° de cat : 60315-1-PBS

Synonymes

SRC, pp60c-src, p60-Src, EC:2.7.10.2, cSRC

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Galerie de données de validation

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  WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  WB analysis using 60315-1-Ig (same clone as 60315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60315-1-Ig (same clone as 60315-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  WB analysis of CHO using 60315-1-Ig (same clone as 60315-1-PBS)

  CHO cells were subjected to SDS PAGE followed by western blot with 60315-1-Ig (c-SRC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60315-1-Ig (c-SRC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60315-1-Ig (same clone as 60315-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using c-SRC antibody (60315-1-Ig, Clone: 5E10C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60315-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60315-1-PBS cible c-SRC dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris, Hamster

• Réactivité: Humain, rat, souris, Hamster
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: c-SRC Protéine recombinante Ag22306
• Nom complet: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC011566
• Symbole du gène: SRC
• Identification du gène (NCBI): 6714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

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