Anticorps Polyclonal de lapin anti-cGAS

• Phare
• Validé par KD/KO

cGAS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(25)

N° de cat : 29958-1-AP

Synonymes

m cGAS, Mb21d1, mcGAS, m-cGAS

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Galerie de données de validation

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  WB analysis using 29958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29958-1-AP (cGAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 29958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using cGAS antibody (29958-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules RAW 264.7, cellules Sp2/0
• Résultats positifs en IHC: tissu placentaire humain, tissu pulmonaire de souris, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

29958-1-AP cible cGAS dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: cGAS Protéine recombinante Ag31677
• Nom complet: RIKEN cDNA E330016A19 gene
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC145651
• Symbole du gène: cGAS
• Identification du gène (NCBI): 214763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

Publications

Species	Application	Title
rat,human	WB	Adv Sci (Weinh); Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury; Authors - Qiaohui Chen
mouse	WB	J Adv Res; TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway; Authors - Kai Sun
mouse	WB	Cell Rep; Exercise-induced adipokine Nrg4 alleviates MASLD by disrupting hepatic cGAS-STING signaling; Authors - Min Chen ; KD Validated
mouse	WB,IF	Respir Res; YAP/TAZ-mediated nuclear membrane rupture in promoting senescence of skeletal muscle associated with COPD; Authors - Ge Gong
mouse	WB	Naunyn Schmiedebergs Arch Pharmacol; Decursin protects against DSS-induced experimental colitis in mice by inhibiting the cGAS-STING signaling pathway; Authors - Jiamin Wang
mouse	WB	Food Chem Toxicol; Triptolide exposure triggers ovarian inflammation by activating cGAS-STING pathway and decrease oocyte quality in mouse; Authors - Si-Yao Cheng

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