Anticorps Polyclonal de lapin anti-cGAS

• Phare
• Validé par KD/KO

cGAS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29958-1-PBS

Synonymes

m cGAS, Mb21d1, mcGAS, m-cGAS

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Galerie de données de validation

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  WB analysis using 29958-1-AP (same clone as 29958-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29958-1-AP (cGAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 29958-1-AP (same clone as 29958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using cGAS antibody (29958-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29958-1-PBS cible cGAS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: cGAS Protéine recombinante Ag31677
• Nom complet: RIKEN cDNA E330016A19 gene
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC145651
• Symbole du gène: cGAS
• Identification du gène (NCBI): 214763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

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