Anticorps Monoclonal anti-cGAS

cGAS Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2G2C5

N° de cat : 68640-1-Ig

Synonymes

2'3'-cGAMP synthase, 2G2C5, C6orf150, cGAMP synthase, EC:2.7.7.86

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Galerie de données de validation

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  WB analysis using 68640-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68640-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68640-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68640-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (68640-1-Ig, Clone: 2G2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: Karpas-299 cells, cellules A431, cellules MDA-MB-231, cellules NCI-H1299
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

68640-1-Ig cible cGAS dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: cGAS Protéine recombinante Ag30074
• Nom complet: chromosome 6 open reading frame 150
• Masse moléculaire calculée: 496 aa, 54 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC113608
• Symbole du gène: cGAS
• Identification du gène (NCBI): 115004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Publications

Species	Application	Title
mouse	WB	Environ Pollut; Disturbance of Mitochondrial Dynamics Led to Spermatogenesis Disorder in Mice Exposed to Polystyrene Micro- and Nanoplastics; Authors - Moxuan Zhao
mouse	WB	J Inflamm Res; Study on the Mechanism of Artesunate in Modulating AR Epithelial Injury and Th2-Type Inflammatory Status; Authors - Youwei Bao

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