Anticorps Monoclonal anti-cGAS

cGAS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G2C5

N° de cat : 68640-1-PBS

Synonymes

2'3'-cGAMP synthase, 2G2C5, C6orf150, cGAMP synthase, EC:2.7.7.86

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Galerie de données de validation

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  WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68640-1-Ig (same clone as 68640-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (68640-1-Ig, Clone: 2G2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68640-1-PBS cible cGAS dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: cGAS Protéine recombinante Ag30074
• Nom complet: chromosome 6 open reading frame 150
• Masse moléculaire calculée: 496 aa, 54 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC113608
• Symbole du gène: cGAS
• Identification du gène (NCBI): 115004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

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