Anticorps Recombinant de lapin anti-cGAS

cGAS Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240964E2

N° de cat : 84045-1-PBS

Synonymes

240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS

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Galerie de données de validation

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  WB analysis using 84045-1-RR (same clone as 84045-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84045-1-RR (cGAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 84045-1-RR (same clone as 84045-1-PBS)

  IP result of anti-cGAS (IP:84045-1-RR, 4ug; Detection:84045-1-RR 1:600) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 84045-1-RR (same clone as 84045-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using cGAS antibody (84045-1-RR, Clone: 240964E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84045-1-PBS cible cGAS dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: cGAS Protéine recombinante Ag31677
• Nom complet: RIKEN cDNA E330016A19 gene
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC145651
• Symbole du gène: cGAS
• Identification du gène (NCBI): 214763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

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