Anticorps Recombinant de lapin anti-cGAS

cGAS Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240964E2

N° de cat : 84045-1-RR

Synonymes

240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS

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Galerie de données de validation

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  WB analysis using 84045-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84045-1-RR (cGAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 84045-1-RR

  IP result of anti-cGAS (IP:84045-1-RR, 4ug; Detection:84045-1-RR 1:600) with NIH/3T3 cells lysate 1920 ug.

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  IF Staining of RAW 264.7 using 84045-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using cGAS antibody (84045-1-RR, Clone: 240964E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C2C12, cellules RAW 264.7
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

84045-1-RR cible cGAS dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: cGAS Protéine recombinante Ag31677
• Nom complet: RIKEN cDNA E330016A19 gene
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC145651
• Symbole du gène: cGAS
• Identification du gène (NCBI): 214763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Int Immunopharmacol; cGAS-STING mediates neutrophil extracellular traps-induced EMT in myositis-associated interstitial lung disease: STING as a potential therapeutic target; Authors - Yingyue Feng

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