Anticorps Monoclonal anti-cIAP1

cIAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H3F1

N° de cat : 66626-1-PBS

Synonymes

API1, BIRC2, MIHB, RNF48, 1H3F1

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Galerie de données de validation

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  WB analysis using 66626-1-Ig (same clone as 66626-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  WB analysis using 66626-1-Ig (same clone as 66626-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66626-1-Ig (cIAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66626-1-PBS cible cIAP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: cIAP1 Protéine recombinante Ag21398
• Nom complet: baculoviral IAP repeat-containing 2
• Masse moléculaire calculée: 618 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC016174
• Symbole du gène: cIAP1
• Identification du gène (NCBI): 329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).

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