Anticorps Recombinant de lapin anti-Cardiac Troponin T

Cardiac Troponin T Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250230B1

N° de cat : 81638-3-PBS

Synonymes

cTnT, TNNT2, Troponin T, cardiac muscle

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Galerie de données de validation

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  WB analysis using 81638-3-RR (same clone as 81638-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81638-3-RR (cTnT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81638-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 81638-3-RR (same clone as 81638-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81638-3-RR (cTnT antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81638-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 81638-3-RR (same clone as 81638-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81638-3-RR (cTnT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81638-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 81638-3-RR (same clone as 81638-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using cTnT antibody (81638-3-RR, Clone: 250230B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81638-3-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 81638-3-RR (same clone as 81638-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using cTnT antibody (81638-3-RR, Clone: 250230B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81638-3-PBS in a different storage buffer formulation.

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Informations sur le produit

81638-3-PBS cible Cardiac Troponin T dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cardiac Troponin T Protéine recombinante Ag7716
• Nom complet: troponin T type 2 (cardiac)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34-40 kDa
• Numéro d’acquisition GenBank: BC002653
• Symbole du gène: Cardiac Troponin T
• Identification du gène (NCBI): 7139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

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