Anticorps Recombinant de lapin anti-calreticulin

calreticulin Recombinant Antibody for WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2B17

N° de cat : CL750-80017

Synonymes

CALR, Calregulin, 2B17

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Galerie de données de validation

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  WB analysis using CL750-80017

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-80017 (calreticulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using CL750-80017

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated calreticulin Recombinant antibody (CL750-80017, Clone:2B17)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Jurkat using CL750-80017

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated calreticulin Recombinant antibody (CL750-80017, Clone:2B17)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562
• Résultats positifs en FC (Intra): cellules K-562, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-80017 cible calreticulin dans les applications de WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: calreticulin Protéine recombinante Ag26064
• Nom complet: calreticulin
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC002500
• Symbole du gène: Calreticulin
• Identification du gène (NCBI): 811
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

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