Anticorps Polyclonal de lapin anti-Cleaved Caspase 3
Cleaved Caspase 3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (4)
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 25128-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules Jurkat, |
Résultats positifs en IHC | tissu cérébral de souris, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 254 publications below |
IHC | See 30 publications below |
IF | See 21 publications below |
Informations sur le produit
25128-1-AP cible Cleaved Caspase 3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, bovin, Chèvre, Humain, poulet, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | caspase 3, apoptosis-related cysteine peptidase |
Masse moléculaire calculée | 32 kDa |
Poids moléculaire observé | 17-25 kDa |
Numéro d’acquisition GenBank | NM_032991 |
Symbole du gène | Caspase 3 |
Identification du gène (NCBI) | 836 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).
Protocole
Product Specific Protocols | |
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WB protocol for Cleaved Caspase 3 antibody 25128-1-AP | Download protocol |
IHC protocol for Cleaved Caspase 3 antibody 25128-1-AP | Download protocol |
IF protocol for Cleaved Caspase 3 antibody 25128-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Hazard Mater Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: Its potential role in PCOS | ||
Small A Responsive Nanorobot Modulates Intracellular Zinc Homeostasis to Amplify Mitochondria-Targeted Phototherapy | ||
Redox Biol DNAJC12 causes breast cancer chemotherapy resistance by repressing doxorubicin-induced ferroptosis and apoptosis via activation of AKT | ||
J Adv Res TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway | ||
Adv Healthc Mater Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation | ||
Sci Total Environ Nasal instillation of polystyrene nanoplastics induce lung injury via mitochondrial DNA release and activation of the cyclic GMP-AMP synthase-stimulator of interferon genes-signaling cascade |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Vignesh (Verified Customer) (09-19-2025) |
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