Anticorps Polyclonal de lapin anti-Cleaved Caspase 3

Cleaved Caspase 3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(277)

Avis (1)

N° de cat : 25128-1-AP

Synonymes

CASP3, Caspase3, CASP 3, CASP-3, Caspase 3

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Galerie de données de validation

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  WB analysis using 25128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 25128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 25128-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (25128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 254 publications below
• IHC: See 30 publications below
• IF: See 21 publications below

Informations sur le produit

25128-1-AP cible Cleaved Caspase 3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 17-25 kDa
• Numéro d’acquisition GenBank: NM_032991
• Symbole du gène: Caspase 3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publications

Species	Application	Title
human	WB,IHC	J Hazard Mater; Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: Its potential role in PCOS; Authors - Hanxi Yu
human	WB	Small; A Responsive Nanorobot Modulates Intracellular Zinc Homeostasis to Amplify Mitochondria-Targeted Phototherapy; Authors - Xinyuan Zhou
human	WB	Redox Biol; DNAJC12 causes breast cancer chemotherapy resistance by repressing doxorubicin-induced ferroptosis and apoptosis via activation of AKT; Authors - Mengjia Shen
mouse	WB	J Adv Res; TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway; Authors - Kai Sun
mouse	WB,IHC	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
mouse	WB	Sci Total Environ; Nasal instillation of polystyrene nanoplastics induce lung injury via mitochondrial DNA release and activation of the cyclic GMP-AMP synthase-stimulator of interferon genes-signaling cascade; Authors - Jing Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vignesh (Verified Customer) (09-19-2025)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 231cells

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