Anticorps Polyclonal de lapin anti-Cleaved Caspase 3

Cleaved Caspase 3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(277)

N° de cat : 25128-1-AP

Synonymes

CASP3, Caspase3, CASP 3, CASP-3, Caspase 3



Applications testées

Résultats positifs en WBcellules Jurkat,
Résultats positifs en IHCtissu cérébral de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

25128-1-AP cible Cleaved Caspase 3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, bovin, Chèvre, Humain, poulet, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet caspase 3, apoptosis-related cysteine peptidase
Masse moléculaire calculée 32 kDa
Poids moléculaire observé17-25 kDa
Numéro d’acquisition GenBankNM_032991
Symbole du gène Caspase 3
Identification du gène (NCBI) 836
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Protocole

Product Specific Protocols
WB protocol for Cleaved Caspase 3 antibody 25128-1-APDownload protocol
IHC protocol for Cleaved Caspase 3 antibody 25128-1-APDownload protocol
IF protocol for Cleaved Caspase 3 antibody 25128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Hazard Mater

Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: Its potential role in PCOS

Authors - Hanxi Yu
humanWB

Small

A Responsive Nanorobot Modulates Intracellular Zinc Homeostasis to Amplify Mitochondria-Targeted Phototherapy

Authors - Xinyuan Zhou
humanWB

Redox Biol

DNAJC12 causes breast cancer chemotherapy resistance by repressing doxorubicin-induced ferroptosis and apoptosis via activation of AKT

Authors - Mengjia Shen
mouseWB

J Adv Res

TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway

Authors - Kai Sun
mouseWB,IHC

Adv Healthc Mater

Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation

Authors - Ning Wang
mouseWB

Sci Total Environ

Nasal instillation of polystyrene nanoplastics induce lung injury via mitochondrial DNA release and activation of the cyclic GMP-AMP synthase-stimulator of interferon genes-signaling cascade

Authors - Jing Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Vignesh (Verified Customer) (09-19-2025)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: 231cells
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