Anticorps Polyclonal de lapin anti-Cyclin T2

Cyclin T2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21860-1-PBS

Synonymes

CCNT2, Cyclin T, Cyclin-T2, CycT2

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Galerie de données de validation

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  WB analysis using 21860-1-AP (same clone as 21860-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21860-1-AP (cyclin T2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21860-1-AP (same clone as 21860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 4010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21860-1-AP (same clone as 21860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21860-1-AP (same clone as 21860-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21860-1-AP (cyclin T2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21860-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21860-1-PBS cible Cyclin T2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin T2 Protéine recombinante Ag16514
• Nom complet: cyclin T2
• Masse moléculaire calculée: 730 aa, 81 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC114366
• Symbole du gène: Cyclin T2
• Identification du gène (NCBI): 905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin T2 (CCNT2) belongs to the cyclin family. CCNT2 is regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II)  (PMID: 9499409, PMID: 15563843). Cyclin T2 is expressed at a relatively high level in freshly isolated monocytes and its level remains constant when the cells are induced to undergo macrophage differentiation (PMID: 21791050). In contrast, Cyclin T1 is expressed at low levels in monocytes and it is strongly up-regulated by a post-transcriptional mechanism when the cells are induced to differentiate to macrophages.

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