Anticorps Recombinant de lapin anti-dsRNA

dsRNA Recombinant Antibody for IF/ICC, FC (Intra), Dot Blot, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

n/a

Applications

IF/ICC, FC (Intra), Dot Blot, ELISA

Conjugaison

Non conjugué

CloneNo.

250708A2

N° de cat : 85780-2-RR

Synonymes

250708A2

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Galerie de données de validation

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  IF Staining of Vero using 85780-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Live vaccines of TGEV and PEDV-infected Vero cells using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Transfected Vero using 85780-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected Vero using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Transfected Vero using 85780-2-RR

  1x10^6 Transfected Vero were intracellularly stained with 0.25 ug dsRNA Recombinant antibody (85780-2-RR, Clone:250708A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Dot Blot experiment using 85780-2-RR

  Dot blot analysis was used to confirm the specificity of 85780-2-RR anti-dsRNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row.

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  Dot Blot experiment of Poly(I:C) using 85780-2-RR

  Dot blot analysis was used to confirm the specificity of 85780-2-RR dsRNA antibody. samples were spotted onto NC and probed with antibody at 1 µg/ml.The amount of sample (μg/mL) spotted is indicated next to each row.

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Applications testées

• Résultats positifs en IF/ICC: Live vaccines of TGEV and PEDV-infected Vero cells, Transfected Vero
• Résultats positifs en FC (Intra): Transfected Vero,
• Résultats positifs en Dot Blot: N/A, Poly(I:C)

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• DOT BLOT: DOT BLOT : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85780-2-RR cible dsRNA dans les applications de IF/ICC, FC (Intra), Dot Blot, ELISA et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: dsRNA
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

dsRNA is formed by the base pairing of two RNA strands, creating a double helix structure similar to DNA, but with some differences. dsRNA can be generated through various mechanisms. In the context of viral infections, it can be derived from the genomes of dsRNA viruses, replication intermediates of single-stranded RNA viruses, or bidirectional transcription of certain DNA viruses. Additionally, aberrant splicing or transcriptional processes may lead to the formation of dsRNA structures. dsRNA is recognized as a pathogen-associated molecular pattern (PAMP) by the immune system. It can trigger a series of immune responses in cells, including the activation of antiviral innate immune pathways.

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