Anticorps Polyclonal de lapin anti-Dynamitin (1-83 AA)

Dynamitin (1-83 AA) Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP,ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 10031-2-AP

Synonymes

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50

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Galerie de données de validation

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  WB analysis of mouse brain using 10031-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10031-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10031-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10031-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10031-2-AP

  IP Result of anti-Dynamitin (IP:10031-2-AP, 3ug; Detection:10031-2-AP 1:800) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu pulmonaire de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10031-2-AP cible Dynamitin (1-83 AA) dans les applications de WB, IP,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: dynactin 2 (p50)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC000718
• Symbole du gène: DCTN2
• Identification du gène (NCBI): 10540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 59aa of full length p50 dynamitin.

Publications

Species	Application	Title
mouse	WB	Nat Med; PMID: 19838201; Authors - Cheng Zhiyong Z

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

X (Verified Customer) (10-29-2020)

A very good p50 antibody to detect p50 at the righ MW by Western.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain/human brain lysates

Dynamitin (1-83 AA) Antibody Western Blot validation (1:2000 dilution) in mouse brain/human brain lysates (Cat no:10031-2-AP)