Anticorps Polyclonal de lapin anti-Dynamitin (1-86 AA)

Dynamitin (1-86 AA) Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

N° de cat : 10032-1-AP

Synonymes

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50

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Galerie de données de validation

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  WB analysis of mouse lung using 10032-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10032-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10032-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10032-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10032-1-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu cardiaque humain, tissu cérébral humain, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10032-1-AP cible Dynamitin (1-86 AA) dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dynamitin (1-86 AA) Protéine recombinante Ag0029
• Nom complet: dynactin 2 (p50)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: AY189155
• Symbole du gène: DCTN2
• Identification du gène (NCBI): 10540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 86aa of full length p50 dynamitin.