Anticorps Monoclonal anti-fetuin-B

fetuin-B Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2H7D11

N° de cat : 67002-1-Ig

Synonymes

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

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Galerie de données de validation

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  WB analysis of human blood using 67002-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 67002-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit serum using 67002-1-Ig

  rabbit serum were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 67002-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67002-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67002-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67002-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: plasma humain, sang humain, Sanguin humain
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

67002-1-Ig cible fetuin-B dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, Lapin

• Réactivité: Humain, Lapin
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: fetuin-B Protéine recombinante Ag12463
• Nom complet: fetuin B
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC074734
• Symbole du gène: Fetuin B
• Identification du gène (NCBI): 26998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

Publications

Species	Application	Title
human,mouse	WB	J Agric Food Chem; Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of De Novo Lipogenesis.; Authors - Yan Xing
mouse	WB	J Proteome Res; Novel Regulators of Retina Neovascularization: A Proteomics Approach.; Authors - Manhong Xu
mouse	WB	Front Pharmacol; Delineation of renal protein profiles in aristolochic acid I-induced nephrotoxicity in mice by label-free quantitative proteomics; Authors - Xinhui Liu
human	IHC	Mol Cell Proteomics; Proteomic analysis of human follicular fluid-derived exosomes reveals that insufficient folliculogenesis in aging women is associated with infertility; Authors - Zhen Liu

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