Anticorps Monoclonal anti-fetuin-B

fetuin-B Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H7D11

N° de cat : 67002-1-PBS

Synonymes

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

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Galerie de données de validation

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  WB analysis of human blood using 67002-1-Ig (same clone as 67002-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 67002-1-Ig (same clone as 67002-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit serum using 67002-1-Ig (same clone as 67002-1-PBS)

  rabbit serum were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67002-1-PBS cible fetuin-B dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin

• Réactivité: Humain, Lapin
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: fetuin-B Protéine recombinante Ag12463
• Nom complet: fetuin B
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC074734
• Symbole du gène: Fetuin B
• Identification du gène (NCBI): 26998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

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