Anticorps Polyclonal de lapin anti-Gamma Catenin

Gamma Catenin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 27872-1-AP

Synonymes

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

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Galerie de données de validation

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  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (Gamma Catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 27872-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27872-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27872-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27872-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27872-1-AP (gamma catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 27872-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 27872-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MCF-7 using 27872-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27872-1-AP (gamma catenin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HUVEC, cellules MCF-7, cellules T-47D, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

27872-1-AP cible Gamma Catenin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Gamma Catenin Protéine recombinante Ag27394
• Nom complet: junction plakoglobin
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC000441
• Symbole du gène: Gamma Catenin
• Identification du gène (NCBI): 3728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Oncol (Dordr); The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.; Authors - Yizhi Xiao
human	WB,IF	BMC Cancer; Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines; Authors - Jueyao Zou
human	WB,IF	Int J Biol Macromol; Frameshift variants in the C-terminal of CTNNB1 cause familial exudative vitreoretinopathy by AXIN1-mediated ubiquitin-proteasome degradation condensation; Authors - Yining Liu

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