Anticorps Polyclonal de lapin anti-gephyrin

gephyrin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12681-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12681-1-AP (same clone as 12681-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12681-1-AP (gephyrin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

  IP result of anti-gephyrin (IP:12681-1-AP, 4ug; Detection:12681-1-AP 1:700) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 12681-1-AP (gephyrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12681-1-PBS cible gephyrin dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: gephyrin Protéine recombinante Ag3370
• Nom complet: gephyrin
• Masse moléculaire calculée: 769 aa, 83 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: BC030016
• Symbole du gène: GPHN
• Identification du gène (NCBI): 10243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.

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