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Anticorps Polyclonal de lapin anti-gp130/IL6ST

gp130/IL6ST Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30591-1-PBS

Synonymes

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using gp130/IL6ST Polyclonal antibody (30591-1-AP)

    WB analysis using 30591-1-AP (same clone as 30591-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30591-1-AP (gp130/IL6ST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30591-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using gp130/IL6ST Polyclonal antibody (30591-1-AP)

    IF Staining of HepG2 using 30591-1-AP (same clone as 30591-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using gp130/IL6ST antibody (30591-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30591-1-PBS in a different storage buffer formulation.

Informations sur le produit

30591-1-PBS cible gp130/IL6ST dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Protéine recombinante
Nom complet interleukin 6 signal transducer (gp130, oncostatin M receptor)
Masse moléculaire calculée 104 kDa
Poids moléculaire observé130-150 kDa
Numéro d’acquisition GenBankNM_002184.3
Symbole du gène IL6ST
Identification du gène (NCBI) 3572
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).

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