Anticorps Recombinant de lapin anti-gp130/IL6ST

gp130/IL6ST Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242236E1

N° de cat : 84794-4-RR

Synonymes

242236E1, CD130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta

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Galerie de données de validation

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  WB analysis using 84794-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84794-4-RR (gp130/IL6ST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84794-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84794-4-RR against Mouse gp130/IL6ST were performed. The affinity constant is 89.7 pM.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C2C12, cellules Neuro-2a

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84794-4-RR cible gp130/IL6ST dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: gp130/IL6ST Protéine recombinante Eg1631
• Nom complet: interleukin 6 signal transducer
• Masse moléculaire calculée: 102kDa, 917aa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_010560.3
• Symbole du gène: Il6st
• Identification du gène (NCBI): 16195
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines(PMID: 39414364), including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin (PMID: 24099136). This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells. GP130 was originally discovered in 1978 and was successfully cloned and expressed in 1990, leading to a deeper understanding of cytokine signaling pathways (PMID: 308233).

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