Anticorps Recombinant de lapin anti-gp130/IL6ST

gp130/IL6ST Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242292C9

N° de cat : 98121-2-RR

Synonymes

IL6ST, 242292C9, CD130, Glycoprotein 130, GP130

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocyte using 98121-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit RecAb (98121-2-RR, Clone: 242292C9) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using 98121-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit RecAb (98121-2-RR, Clone: 242292C9)(red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98121-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98121-2-RR against Human gp130/IL6ST were performed. The affinity constant is 2.21 nM.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98121-2-RR cible gp130/IL6ST dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: gp130/IL6ST Protéine recombinante Eg0654
• Nom complet: interleukin 6 signal transducer (gp130, oncostatin M receptor)
• Masse moléculaire calculée: 104 kDa
• Numéro d’acquisition GenBank: NM_002184.3
• Symbole du gène: IL6ST
• Identification du gène (NCBI): 3572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).

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