Anticorps Monoclonal anti-hIST1

hIST1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E12A7

N° de cat : 66989-1-PBS

Synonymes

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

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Galerie de données de validation

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  WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66989-1-Ig (same clone as 66989-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66989-1-Ig (same clone as 66989-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 66989-1-Ig (same clone as 66989-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66989-1-PBS cible hIST1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: hIST1 Protéine recombinante Ag28364
• Nom complet: KIAA0174
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC000430
• Symbole du gène: hIST1
• Identification du gène (NCBI): 9798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.

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