Anticorps Polyclonal de lapin anti-hnRNP-E1

• Phare
• Validé par KD/KO

hnRNP-E1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 14523-1-AP

Synonymes

Alpha CP1, hnRNP E1, hnRNP X, hnRNP-E1, HNRPE1, HNRPX, PCBP1, poly(rC) binding protein 1

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Galerie de données de validation

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  WB analysis using 14523-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14523-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14523-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14523-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14523-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14523-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14523-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 14523-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A431 using 14523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules HepG2, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules A431, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

14523-1-AP cible hnRNP-E1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: hnRNP-E1 Protéine recombinante Ag6007
• Nom complet: poly(rC) binding protein 1
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC039742
• Symbole du gène: PCBP1
• Identification du gène (NCBI): 5093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.

Publications

Species	Application	Title
mouse	WB,IF	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
human	IF	Nucleic Acids Res; G-Quadruplex binding enantiomers show chiral selective interactions with human telomere.; Authors - Jiasi Wang
human	IHC	Bioengineered; PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma.; Authors - Kaiming Peng
rat	WB,IHC	Pathol Int; PCBP2 knockdown promotes ferroptosis in malignant mesothelioma.; Authors - Lin Yue ; KD Validated
mouse	WB	Mol Cell; Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.; Authors - David Colognori
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S. H. (Verified Customer) (02-24-2020)

we are happy for the result we got.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000x dilution
• Cell Tissue Type: 293T CELLS

hnRNP-E1 Antibody Western Blot, validation (1000x dilution dilution) in 293T CELLS (Cat no:14523-1-AP)