Anticorps Monoclonal anti-human IgD

human IgD Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D1B12

N° de cat : 67538-1-PBS

Synonymes

IGHD, IgD, 1D1B12

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Galerie de données de validation

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  WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

  human plasma (0.1 ul) were subjected to SDS PAGE followed by western blot with 67538-1-Ig (human IgD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IGHD antibody (67538-1-Ig, Clone: 1D1B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67538-1-PBS cible human IgD dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human IgD Protéine recombinante Ag9614
• Nom complet: immunoglobulin heavy constant delta
• Masse moléculaire calculée: 573 aa, 63 kDa
• Poids moléculaire observé: 55-70 kDa
• Numéro d’acquisition GenBank: BC021276
• Symbole du gène: IGHD
• Identification du gène (NCBI): 3495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .

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