Anticorps Monoclonal anti-human IgM

human IgM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D10B10

N° de cat : 66484-1-PBS

Synonymes

IGHM, IgM, 2D10B10, MU, VH

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Galerie de données de validation

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  WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

  0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

  0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:3200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:3200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66484-1-Ig (IGHM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66484-1-PBS cible human IgM dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human IgM Protéine recombinante Ag1459
• Nom complet: immunoglobulin heavy constant mu
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC009851
• Symbole du gène: IGHM
• Identification du gène (NCBI): 3507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).

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